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研究發現血漿游離DNA測序可預測基因表達

關鍵詞:血漿游離DNA 基因表達  來源:寶芝林大藥房  發布時間:2016-09-01 08:16:55

JINRI,LAIZIAODILIDEKEXUEJIAZAIYIXIANGYANJIUZHONGFAXIAN,XUEJIANGZHONGXUNHUANYOULIDNA(cfDNA)KEYIWEIJIYINBIAODADEYUCETIGONGXIANSUO。GAIYANJIUZUIXINJIEGUOYIFABIAOZAI《Nature Genetics》SHANG。YANJIUYUANLIYONGYOUHUOXINGHESHIHUODEZHUANLUQISHIWEIDIANSHANGYOUDEHEXIAOTICHAYI,KAIFACHUYIZHONGLIYONGcfDNADECEXUSHENDUMOSHIGUJIJIYINBIAODADEFANGFA。YANJIURENYUANLIYONG100DUOMINGJIANKANGRENDEXUEYEYANGBENYANFACHULEZHEZHONGFANGFA,BINGYONGGAIFANGFADUILINGWAISHUBAIMINGZHUANYIAIHUANZHEDEYANGBENJINXINGLEFENXI。

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斗牛棋牌WENZHANGTONGXUNZUOZHE、GELACIYIKEDAXUERENLEIYICHUANXUEYANJIUYUANMichael SpeicherDENGRENXIEDAO,“WOMENLIYONGBAOKUOZHENGGEQIDONGZIQUYUZAINEIDEQUANJIYINZUCEXUSHUJU,LAIQUEDINGJIYINDEBIAODAZHUANGTAI。WOMENDEYANJIUWEILIJIESHIFANGYOULIDNADEXIBAOJIYINZUTIGONGLEXINDESILU,YEWEIcfDNADEFENXITIGONGLEGENGDUOXUANZE。”

LIYONGcfDNADECEXUSHENDUGUJIJIYINBIAODA

DABUFENPIAOFUZAIXUEYEZHONGDEcfDNADOUSHIDIAOWANGXIBAODEYICHUANWUZHI,BAOKUOHEXIAOTIZHONGYUDANBAIFUHETIJIEHEDEDNA。YINCI,YANJIUXIAOZURENWEIKEYILIYONGDNACEXUSHENDUJIESHIYUHUOYUEDEJIYINZHUANLUXIANGGUANDEHEXIAOTIYINJI。

斗牛棋牌YIWANGDEWEIQIUJUNHESUANMEI(MNase)FENXIFAXIAN,JIYINZHUANLUQISHIWEIDIANSHANGYOUYOULIZAIHEXIAOTIZHIWAIDEYANSHENQUYUYEBEIZHUANLU。

KAOLVDAOZHEYIDIAN,YANJIURENYUANSHOUXIANTAOLUNLEDINGWEIZAIQIDONGZIQUDEYUBIAODAXIANGGUANDEHEXIAOTISHIFOUJUYOUZAIcfDNAZHONGBEIJIANCEDAODEMOSHI,RUGUOYOU,NEIMEZHEZHONGBIAOZHISHIFOUNENGFANYINGJIYINBIAODA。CONGZHEXIEWENTICHUFA,YANJIUXIAOZUKUODAFENXIXUNZHAOBIAODAPU,LAIBANGZHUZAIAIZHENGHUANZHEDEXUEYEYANGBENZHONGXUNZHAOAIZHENGQUDONGJIYIN。

YANJIURENYUANXIANSHOUJILE179FENXUEYANG,LAIZI50MINGJIANKANGNANXINGHE54MINGJIANKANGNVXING,LIYONGIlluminaMiSeqHENextSeqCEXUYIJINXINGSHUANGDUANCEXU(paired-end sequencing),XUNZHAOXUEJIANGZHONGYUHEXIAOTIXIANGGUANDEHEDNA。TAMENYOUSHENGCHENGLEDANDUANCEXU(single-end sequencing)SHUJU,JIEHEYIYOUDEMNaseTUPUHELAIZIENCODEJIFANTOM5JIHUADEJIYINBIAODASHUJU,LAIPINGGUZHUANLUQISHIWEIDIANZHOUWEIDECEXUSHENDUMOSHI。GAIXINXIKEYONGLAIKAIFAYIZHONGCONGcfDNACEXUSHENDUZHONGYUCEJIYINBIAODADESUANFA。

研究人員對來自兩個乳腺癌患者血樣的循環腫瘤DNA進行全基因組測序,證明了這種方法可以識別基因表達和拷貝數信息(與原發性腫瘤樣本RNA測序數據一致),隨后他們進一步對426份來自轉移性結腸癌、乳腺癌、肺癌或前列腺癌患者的血樣進行研究,獲得基因表達和拷貝數信息。

YANJIURENYUANBIAOSHI,ZHEZHONGFANGFATEBIESHIYONGYUSHIBIEYINGXIANGAIZHENGQUDONGJIYINDEKUOZENG,DANKENENGBUSHIYONGYUPINGGUWEIXIAOCANLIUBING(minimal residual disease,MRD)DEXUNHUANZHONGLIUDNA。

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